COLD ATMOSPHERIC PLASMA TREATMENT MODULATES THE EXPRESSION OF cdk1, tnfα AND tp53 GENES IN HUMAN OSTEOSARCOMA CELLS

Osteosarcoma (OS), a malignancy primarily affecting children and adolescents, is the most frequently encountered malignant, non-hematologic, bone tumor. Despite gradual improvement of survival rates, management this disease remains problematic due to challenges such metastasis development, its heterogeneous characteristics, resistance cytostatic drugs. Cold atmospheric plasma (CAP), partially ionized gas operating at near room temperature, which comprised free carriers, excited or neutral molecules, active radicals capable initiating diverse physical phenomena chemical reactions represent new innovative potential solution in cancer therapy. The aim study was evaluate capacity CAP produced by our custom-build source induce cytotoxic effects HOS cells analyze post-treatment modulations cdk1, tnfα tp53 genes expression. Direct indirect treatments effectiveness on were evaluated MTT assay regulation interest expression carried out RT-qPCR analysis. Cell viability analysis revealed strong effect direct treatment cells, while resulted slight decrease viability. modulates all analyzed genes, both 2- 24-hours post-treatment. In conclusion, have dose-dependent manner up 24 hours Furthermore, induces cell cycle arrest CAP-induced death independent gene.